University of Hyderabad 2012 Phd BIOTECHNOLOGY PART A PAPER

PART A
1. Which one of the following techniques is used for studying genome wide differential gene expression
A.Western blotting
B. Northern blotting
C. DNA microarray
D. Pyro-sequencing of genomes

2.Which of the following BLAS'f programs is used for protein query against a translated nucleotide sequence database
A. Blastp
B. Blastn
C. tblastx
D. Tblastn

3.Which of the following amino acids is least mutable according to the PAM scoring matrix?
A. Alanine
B. Glutamine
C. Methionine
D. Cysteine

4.If the ratio A26sl A2ss nm of a DNA solution is 1 .4, it indicates contamination of DNA
preparation with
A. Carbohydrate
B. RNA
C. Protein
D. Salt


5.The methods used for locating transcription start site are

A. Gel retardation and foot printing
B. Northem analysis and RT-PCR
C. Primer extension and Sl nuclease mapping
D. Cloning of 5' UTRs and DNA Sequencing

6.Which one of the following is a Ml3 based vector
A.pBR322
B.pT7blue
C. pUC 18
D. Cosmid

7.DNA fragment size suitable to cloning in a l" replacement vector
A. 30 kb
B.35 kb
c. 18 kb
D. 53 kb

8.In a yeast two hybrid assay if two test proteins (bait and prey) are interacting with each other, then
A.LacZ reporter gene will be strongly repressed
B.LacZ reporter gene will be expressed
C.LacZ reporter gene expression is repressed by LacI
D.LacZ reporter gene expression is unaffected


9.RNA is susceptible to alkaline hydrolysis, but not DNA because
A. RNA is single stranded and nitrogenous bases are exposed to alkali
B.RNA can form intra base-pairing, but not DNA
C.RNA has 2'-OH group
D. RNA is smaller than DNA

10. Which one of the following has high gene density
A. Human
B.Arabidopsis
C.Drosophila
D. E.coli

I 1. Processivity of DNA polymerase is enhanced by
A. DNA helicase
B. Sliding clamp
C. Topoisomerase
D. RNA helicase

12. Which form of the following ptasmid DNA migrates faster during agarose gel electrophoresis
A. highly super-coiled plasmid DNA
B. relaxed plasmid DNA
C. linear plasmid DNA
D. nicked circular plasmid DNA

13. Which one of the following E.coli sigma subunit is required for transcription of heat inducible genes
A. Sigma 70
B. Sigma 32
C. Sigma54
D. Sigma28

14. Which one of the following is NOT a state function?
A. lntemal energy
B. Heat
C. Enthalpy
D. Entropy

15. Number of vibrational degrees of freedom for ethylene molecule is
A. 18
B. l5
c.12
D.l I

16. Symmetry elements of ammonia molecule contain
A. Cs-axis of symmetry and one centre of symmetry
B. Cl-axis of symmetry and three planes of symmetry
C. G-axis of symmetry and one centre of symmetry
D. Cz-axis of symmetry and two planes of symmetry

17. Volume of a balloon filled with an ideal gas is 5 L, at 1 atm and 25 oC; what will be the volume of the balloon if temperature increases to 35oC?
A.3.57 L
8.4.84 L
c.5.17 t.
D,7.0 L

18. Which of the following sequence is conect, if the molecules are arranged according to their bond energies?
A. Hz> F2> Cl2> Br2
B. Hz> Cl2>F2> Br2
C. Fz> Clz> Brz>Hz
D. Clz> F2> Br2>H2

19. Addition of an inhibitor does not change K^lV**value, if the mechanism of inhibition rs

A. Competitive
B. Uncompetitive
C. Noncompetitive
D. All the above

20. Generally, in a fluorescent experiment, moving the excitation wavelength to higher wavelength,
A. does not affect the emission wavelength
B. blue shifts emission wavelength
C. red shifts emission wavelength
D. depends on the solvent and temperature

21. The chloroplast genome of higher plants contain
A. Twelve genes
B. Hundred and twenty genes
C. Twelve hundred genes
D. Twelve thousand genes

22. The metabolite that causes internal bleeding in mammals
A. Stilbenes
B. Coumarins
C. Ferulic acid
D. Chalcones

23. Denaturation of aprotein involves
A. Partial loss of amino acid sequence
B. Loss of s-helices
C. Loss of p-sheets
D. Loss of 3-D shape and catalytic activity

24. Aparticle moves with a constant velocity parallel to the X-axis. Its angular momentum with respect to the origin,
A. Is zero
B. Remains constant
C. Goes on decreasing
D. Goes on increasing

25.The secondary pacemaker of the heart is known as the:
A. AV node
B. SA node
C. Purkinje Bundle
D. None of the above