Hureh.......,
CSIR has announced net result of December 2014
to know your result visit the page:
CSIR has announced net result of December 2014
to know your result visit the page:
Monday, 30 March 2015
Sunday, 29 March 2015
CSIR further extended the application last date of csir ugc net June 2015 examination
EXTENSION IN LAST DATE FOR DEPOSIT OF FEE & SUBMISSION OF ONLINEAPPLICATION FOR JOINT CSIR-UGC TEST FOR JUNIOR RESEARCH FELLOWSHIP &ELIGIBILITY FOR LECTURESHIP (NET) SCHEDULED ON 21ST JUNE, 2015
Date of close of deposit of fee 31.03.2015
Date of close of On-line submission of Application 01.04.2015
Last date of receipt of duly completed hard copy of onlineapplication in the Examination Unit.07.04.2015
Last date of receipt of duly completed hard copy of online application in the Examination Unit from remote areas.13.04.2015
further details visit CSIR WEBSITE
Friday, 20 March 2015
University of Hyderabad 2012 Phd BIOTECHNOLOGY PART B PAPER
PART B
26.The resting potential of a neuron is dependent on what two ions?
A. lead and calcium ions
B. calcium and phosphate ions
C. sodium and potassium ions
D. potassium and phosphate ions
27. A double stranded DNA showed a positive peak around 260 nm followed by a negative trough -what form of DNA it is?
A. A form
B. B form
C. C form
D. Z form
28. Alpha helix of a protein can be estimated at the following wavelength in CD spectrum
A. 217 nm
B. 190 nm
C.222nm
D. 280nm
29. DNA
A. is more susceptible than.RNA to degradation at high pH
B. can hybridize with other DNA molecules but not with RNA
C. has fewer hydroxyl groups than RNA
D. has catalytic activity
30. Which one of the following radiolabeled compounds is most commonly used for studying DNA synthesis in cultures of Escherichia coli?
A. [3H] thymidine
g. [14C] uridine
c. [Gamma-32P] dATP
d.[alphs-32P]dATP
3l . The Lac repressor
A. Is induced by exposure of a bacterial cellto lactose
B. Uses the sarne promoter as the lacZ gene
C. Changes the shape in the presence of inducer
D. Can form altemate stem-loop structures
32. Following is not a characteristic of the mRNA 5' cap
A. 7-methylguanosine triphosphate
B. 2'-OH methylation
C. 3'-->5'linkage
D. 5'-->5'linkage
33. How (eukaryotic) viruses acquire lipid membranes?
A. The lipids are synthesized by proteins coded by viral genes.
B. The viral capsid acquires the membrane when it leaves the host cell.
C. The viral capsid acquires the membrane when it is assembled inside the host cell.
D. The viral capsid acquires the membrane rvhen it first binds to a host cell.
34. Which of the following is NOT a type of histone modification?
A. Acetylation
B. ADP-ribosylation
C. Methylation
D. Phosphorylation
35. Enzyme which incorporate oxygen into a substrate are called
A. Oxidases
B. Dehydrogenases
C. Oxygenases
D. Hydroperooxidases
36. The bonds that are important in the secondary structure of protein are
A. Hydrophobic interactions
B. Peptide bonds
C. Hydrogen bonds
D. Disulphide bonds
37. The cell wall component of Gram negative bacteria which is mostly associated with'the induction of septic shock.
A. Capsular protein
B. Endotoxin
C. Peptidoglycan
D. Phospholipid
38. Select the principal means by which antigenic shift occurs in influenza A virus.
A. Low fidelity of DNA dependent DNA polymerase
B. Low fidelity of RNA dependent RNA polymerase
C. Low fidelity of reverse transcriptase
D. Reassortment of fragments of the RNA genome
39. Which of the statement about hemoglobin is NOT correct?
A. Number of contacts between two c chains are less than the contacts between o and p chains
B. Increasing pH increases the percentage of oxygen saturation at a given oxygen partial pressure
C. During deoxygenation, hemes of a chains are slightly separated apart and p chains come closer
D. In sickle cell hemoglobin glutamic acid is replaced with valine in the sixth position of p chain
40. By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to:
A. determine an enzyme's specific activity
B. determine the amino acid composition of the protein
C. preserve a protein's native structure and biological activity
D. separate proteins exclusively on the basis of molecular weight
41. One round of Edman degradation of the peptide: H2N-Gly-Arg-*Lys-Phe-As5 COOH would result in which of the following structures or their phenyl isothiocyanate derivatives?
A. H2N-Gly-Arg-COOH + HzN-Lys-Phe-Asp-{OOH
B. H2N-Gly-Arg-Lys-Phe--COOH + Asp
C. H2N-Arg-Lys-Phe-Asp-COOH + Gly
D. H2N-gly-Arg-Lys--COOH + HN-phe-AsSCOOH
42. Aromatic amino acids can be detected by
A. Sakaguchi reaction.
B. Millon-Nasse reaction
C. Hopkins-Cole reaction
D-Xanthoproteic reaction
43. Death of T cells in negative selection occurs because of
A. Low affinity receptors for self MHC
B. High affinity receptors for self MHC
C. Both
D. None
44.The primary activities of complement system DO NOT include
A. Opsonization
B. Cell Lysis
C. Clearance of immune complexes
D. Tissue regeneration
45. Which of the following is used to search protein database using a translated nucleotide query?
A. BLASTp
B. BLASTx
C. tBLASTn
D. tBLASTx
46. Which of the following is the distance based method for phylogenetic analysis?
A. Neighbor-Joining method
B. Maximum Parsimony method
C. Maximum Likelihood method
D. all above metlods
47. BLOSUM is an acronym for
A. Basic substraction matrix
B. Basic local summation matrix
C. Blocks substitution matrix
D. Blocks substituted mutation
48. Which of the following tool is used for molecular structure file conversion?
A. BABEL
B. PHYLIP
C. CLUSTAL
D. MSFC
49. Which of the following is SMILE code for 2-propanol?
A. CCOC-2
B. CC(O)C
C. CCC-2OH
D. CCCO
50. Glutathione and Clutathione conjugates originate from
A. Methionine
B. S-adenosyl methionine
C. Cysteine
D. Cystine
5l . Bioactive endogenous gibberellic acids are rendered inactive by
A. 2B-hydroxylation
B. 3B-hydroxylation
C. 2,38-hydroxylation
D. 2u-hydroxylation
52. One of the following organisms that produces carbapenemase enzyme and termed as a 'superbug'
A. MRSA
B. Clostridium dfficile
C. NDM-1 positive E. coli
D. Yersinia pestis
53' Bacterial pathogen that has co-evolved with its human host
C. C-reactive proteins
D. All of the above
60. Method(s) used to validate protein-protein interaction
A. Immuno-coprecipitation and pull down
B. Biacore analysis
C. Yeast two hybrid assay
D. All of the above
6l. Which of the following is NOT a virulence factor
A. Invasins
B. Adhesins
C. Collectins
D. Flagellins
62. The following DNA sequence assembly tool is used to assemble short reads data.
A. Velvet
B. Mummer
C. VectorNTI
D. Artemis
63. The following is an online genome annotation tool
A. RAST
B. Mg-RAST
C. Annotate -it
D. GeneScan
64. Which of the following techniques is useful in determining the movement of proteins within a nucleus?
A. Electron microscopy
B. Fluorescence recovery after photobleaching (FRAP)
C. Fluorescent in sffa hybridization (FISH)
D. Confocal light microscopy
65' Nonsense-mediated RNA decay (NMD) is a system for the degradation of eukaryotic mRNA molecules with what features?
A. NMD degrades mRNA molecules with stop codons at incorrect positions.
B. NMD degrades mRNA molecules that encode nonfunctional proteins.
C. NMD degrades mRNA molecules that lack a start codon.
D. NMD degrades mRNA molecules that lack a stop codon.
66. Which one of the following antiviral drugs effective against pandemic influenza targets neuraminidase enzyme of the influenza viruses?
A. Oseltamivir
B. Pleconaril
C. Amantadine
D. None of the above
67. Abacterium that can grow by using arsenic instead of phosphorus in its nucleic acids
A. Halomonas strain GFAJ-l
B. T.ymomonas mobilis
C. Pseudoalteromonas atlantica
D. Psuedomonas fluorescens
68. How do you represent C-alpha atom in pDB format?
A. CA
B.C
C. C-alpha
D. cr
69. Genomic analysis of unculturable organisms in a habitat within an ecosystem is called
A. Metagenomics
B. Microbiome analysis
C. Phylogenomics
D. Epigenomics
70. A sequencing technique that is used to identify binding sites of DNA associated proteins
A. Solexa sequencing
B. ChlP-sequencing
C. Pyrosequencing
D. SoLiD sequencing
71. The following transcription factor is said to be uat the cross-roads of life and death,
as it operates upstream to both the proliferative and apoptotic signaling pathways
A. P53
B. NFAT
C. NfKB
D. None of the above
72. Amount of phosphoric acid needed to neutralize 100 g of magnesium hydroxide is
A. Salmonel la paratyphi
B. Yersinia enterocolitica
C. Helicobacter pylori
D. C oryne bacte rium diphtheriae
54. The new science of systems biology aims at?
A. Generation of multi-omics data
B. Making sense out of next generation sequencing data
C' Integration of multi-omics data and mathematical approaches to propose testable biological models relevant in discovery
D. To deliver designer therapeutics and vaccines
55' Which one of the following is connecting link between Photosystem I and photosystem II
A. plastocyanin
B. fenedoxin
C. cytochrome c
D. cytochrome 66/complex
56' Which of the following global profiles is relevant in the development of sero-diagnostic markers?
A. Transcriptome
B. Secretome
C. Virulome
D. Interactome
57. Arbuscular mycorrhizaare formed by fungi of the division
A. Basidiomycota
B. Ascomycota
C. Zygomycota
D. Glomeromycota
58. which one of the following immunoglobulins can readily cross placenta
A. IgM
B.IgG
C. lgA
D. tgE
59. which of the following is/are Nor a component of plant immune system?
A. Pattern recognition receptors (pRR) i
B. NB-LRR
A. ll2 g
B. 168 g
C. 252 g
D. 66.7 g
73. One of the following is NOT a DNA fingerprinting technique
A. Variable number of tandem repeat analysis
B. Restriction fragment length polymorphism analysis
C. Differential display
D. Pulsed Field Gel Electrophoresis
74. Sickle cell anemia provides genetic resistance towards one of the following diseases
A. Diabetes
B. Malaria
C. Tuberculosis
D. Coronary artery diseases
75. Neuronal cell death in Alzheimer's disease is induced by the following factor(s)
A. Amyloid -B
B. Reactive oxygen species
C. Nitric oxide and ER-stress
D. All of the above
26.The resting potential of a neuron is dependent on what two ions?
A. lead and calcium ions
B. calcium and phosphate ions
C. sodium and potassium ions
D. potassium and phosphate ions
27. A double stranded DNA showed a positive peak around 260 nm followed by a negative trough -what form of DNA it is?
A. A form
B. B form
C. C form
D. Z form
28. Alpha helix of a protein can be estimated at the following wavelength in CD spectrum
A. 217 nm
B. 190 nm
C.222nm
D. 280nm
29. DNA
A. is more susceptible than.RNA to degradation at high pH
B. can hybridize with other DNA molecules but not with RNA
C. has fewer hydroxyl groups than RNA
D. has catalytic activity
30. Which one of the following radiolabeled compounds is most commonly used for studying DNA synthesis in cultures of Escherichia coli?
A. [3H] thymidine
g. [14C] uridine
c. [Gamma-32P] dATP
d.[alphs-32P]dATP
3l . The Lac repressor
A. Is induced by exposure of a bacterial cellto lactose
B. Uses the sarne promoter as the lacZ gene
C. Changes the shape in the presence of inducer
D. Can form altemate stem-loop structures
32. Following is not a characteristic of the mRNA 5' cap
A. 7-methylguanosine triphosphate
B. 2'-OH methylation
C. 3'-->5'linkage
D. 5'-->5'linkage
33. How (eukaryotic) viruses acquire lipid membranes?
A. The lipids are synthesized by proteins coded by viral genes.
B. The viral capsid acquires the membrane when it leaves the host cell.
C. The viral capsid acquires the membrane when it is assembled inside the host cell.
D. The viral capsid acquires the membrane rvhen it first binds to a host cell.
34. Which of the following is NOT a type of histone modification?
A. Acetylation
B. ADP-ribosylation
C. Methylation
D. Phosphorylation
35. Enzyme which incorporate oxygen into a substrate are called
A. Oxidases
B. Dehydrogenases
C. Oxygenases
D. Hydroperooxidases
36. The bonds that are important in the secondary structure of protein are
A. Hydrophobic interactions
B. Peptide bonds
C. Hydrogen bonds
D. Disulphide bonds
37. The cell wall component of Gram negative bacteria which is mostly associated with'the induction of septic shock.
A. Capsular protein
B. Endotoxin
C. Peptidoglycan
D. Phospholipid
38. Select the principal means by which antigenic shift occurs in influenza A virus.
A. Low fidelity of DNA dependent DNA polymerase
B. Low fidelity of RNA dependent RNA polymerase
C. Low fidelity of reverse transcriptase
D. Reassortment of fragments of the RNA genome
39. Which of the statement about hemoglobin is NOT correct?
A. Number of contacts between two c chains are less than the contacts between o and p chains
B. Increasing pH increases the percentage of oxygen saturation at a given oxygen partial pressure
C. During deoxygenation, hemes of a chains are slightly separated apart and p chains come closer
D. In sickle cell hemoglobin glutamic acid is replaced with valine in the sixth position of p chain
40. By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to:
A. determine an enzyme's specific activity
B. determine the amino acid composition of the protein
C. preserve a protein's native structure and biological activity
D. separate proteins exclusively on the basis of molecular weight
41. One round of Edman degradation of the peptide: H2N-Gly-Arg-*Lys-Phe-As5 COOH would result in which of the following structures or their phenyl isothiocyanate derivatives?
A. H2N-Gly-Arg-COOH + HzN-Lys-Phe-Asp-{OOH
B. H2N-Gly-Arg-Lys-Phe--COOH + Asp
C. H2N-Arg-Lys-Phe-Asp-COOH + Gly
D. H2N-gly-Arg-Lys--COOH + HN-phe-AsSCOOH
42. Aromatic amino acids can be detected by
A. Sakaguchi reaction.
B. Millon-Nasse reaction
C. Hopkins-Cole reaction
D-Xanthoproteic reaction
43. Death of T cells in negative selection occurs because of
A. Low affinity receptors for self MHC
B. High affinity receptors for self MHC
C. Both
D. None
44.The primary activities of complement system DO NOT include
A. Opsonization
B. Cell Lysis
C. Clearance of immune complexes
D. Tissue regeneration
45. Which of the following is used to search protein database using a translated nucleotide query?
A. BLASTp
B. BLASTx
C. tBLASTn
D. tBLASTx
46. Which of the following is the distance based method for phylogenetic analysis?
A. Neighbor-Joining method
B. Maximum Parsimony method
C. Maximum Likelihood method
D. all above metlods
47. BLOSUM is an acronym for
A. Basic substraction matrix
B. Basic local summation matrix
C. Blocks substitution matrix
D. Blocks substituted mutation
48. Which of the following tool is used for molecular structure file conversion?
A. BABEL
B. PHYLIP
C. CLUSTAL
D. MSFC
49. Which of the following is SMILE code for 2-propanol?
A. CCOC-2
B. CC(O)C
C. CCC-2OH
D. CCCO
50. Glutathione and Clutathione conjugates originate from
A. Methionine
B. S-adenosyl methionine
C. Cysteine
D. Cystine
5l . Bioactive endogenous gibberellic acids are rendered inactive by
A. 2B-hydroxylation
B. 3B-hydroxylation
C. 2,38-hydroxylation
D. 2u-hydroxylation
52. One of the following organisms that produces carbapenemase enzyme and termed as a 'superbug'
A. MRSA
B. Clostridium dfficile
C. NDM-1 positive E. coli
D. Yersinia pestis
53' Bacterial pathogen that has co-evolved with its human host
C. C-reactive proteins
D. All of the above
60. Method(s) used to validate protein-protein interaction
A. Immuno-coprecipitation and pull down
B. Biacore analysis
C. Yeast two hybrid assay
D. All of the above
6l. Which of the following is NOT a virulence factor
A. Invasins
B. Adhesins
C. Collectins
D. Flagellins
62. The following DNA sequence assembly tool is used to assemble short reads data.
A. Velvet
B. Mummer
C. VectorNTI
D. Artemis
63. The following is an online genome annotation tool
A. RAST
B. Mg-RAST
C. Annotate -it
D. GeneScan
64. Which of the following techniques is useful in determining the movement of proteins within a nucleus?
A. Electron microscopy
B. Fluorescence recovery after photobleaching (FRAP)
C. Fluorescent in sffa hybridization (FISH)
D. Confocal light microscopy
65' Nonsense-mediated RNA decay (NMD) is a system for the degradation of eukaryotic mRNA molecules with what features?
A. NMD degrades mRNA molecules with stop codons at incorrect positions.
B. NMD degrades mRNA molecules that encode nonfunctional proteins.
C. NMD degrades mRNA molecules that lack a start codon.
D. NMD degrades mRNA molecules that lack a stop codon.
66. Which one of the following antiviral drugs effective against pandemic influenza targets neuraminidase enzyme of the influenza viruses?
A. Oseltamivir
B. Pleconaril
C. Amantadine
D. None of the above
67. Abacterium that can grow by using arsenic instead of phosphorus in its nucleic acids
A. Halomonas strain GFAJ-l
B. T.ymomonas mobilis
C. Pseudoalteromonas atlantica
D. Psuedomonas fluorescens
68. How do you represent C-alpha atom in pDB format?
A. CA
B.C
C. C-alpha
D. cr
69. Genomic analysis of unculturable organisms in a habitat within an ecosystem is called
A. Metagenomics
B. Microbiome analysis
C. Phylogenomics
D. Epigenomics
70. A sequencing technique that is used to identify binding sites of DNA associated proteins
A. Solexa sequencing
B. ChlP-sequencing
C. Pyrosequencing
D. SoLiD sequencing
71. The following transcription factor is said to be uat the cross-roads of life and death,
as it operates upstream to both the proliferative and apoptotic signaling pathways
A. P53
B. NFAT
C. NfKB
D. None of the above
72. Amount of phosphoric acid needed to neutralize 100 g of magnesium hydroxide is
A. Salmonel la paratyphi
B. Yersinia enterocolitica
C. Helicobacter pylori
D. C oryne bacte rium diphtheriae
54. The new science of systems biology aims at?
A. Generation of multi-omics data
B. Making sense out of next generation sequencing data
C' Integration of multi-omics data and mathematical approaches to propose testable biological models relevant in discovery
D. To deliver designer therapeutics and vaccines
55' Which one of the following is connecting link between Photosystem I and photosystem II
A. plastocyanin
B. fenedoxin
C. cytochrome c
D. cytochrome 66/complex
56' Which of the following global profiles is relevant in the development of sero-diagnostic markers?
A. Transcriptome
B. Secretome
C. Virulome
D. Interactome
57. Arbuscular mycorrhizaare formed by fungi of the division
A. Basidiomycota
B. Ascomycota
C. Zygomycota
D. Glomeromycota
58. which one of the following immunoglobulins can readily cross placenta
A. IgM
B.IgG
C. lgA
D. tgE
59. which of the following is/are Nor a component of plant immune system?
A. Pattern recognition receptors (pRR) i
B. NB-LRR
A. ll2 g
B. 168 g
C. 252 g
D. 66.7 g
73. One of the following is NOT a DNA fingerprinting technique
A. Variable number of tandem repeat analysis
B. Restriction fragment length polymorphism analysis
C. Differential display
D. Pulsed Field Gel Electrophoresis
74. Sickle cell anemia provides genetic resistance towards one of the following diseases
A. Diabetes
B. Malaria
C. Tuberculosis
D. Coronary artery diseases
75. Neuronal cell death in Alzheimer's disease is induced by the following factor(s)
A. Amyloid -B
B. Reactive oxygen species
C. Nitric oxide and ER-stress
D. All of the above
Thursday, 19 March 2015
University of Hyderabad 2012 Phd BIOTECHNOLOGY PART A PAPER
PART A
1. Which one of the following techniques is used for studying genome wide differential gene expression
A.Western blotting
B. Northern blotting
C. DNA microarray
D. Pyro-sequencing of genomes
2.Which of the following BLAS'f programs is used for protein query against a translated nucleotide sequence database
A. Blastp
B. Blastn
C. tblastx
D. Tblastn
3.Which of the following amino acids is least mutable according to the PAM scoring matrix?
A. Alanine
B. Glutamine
C. Methionine
D. Cysteine
4.If the ratio A26sl A2ss nm of a DNA solution is 1 .4, it indicates contamination of DNA
preparation with
A. Carbohydrate
B. RNA
C. Protein
D. Salt
5.The methods used for locating transcription start site are
A. Gel retardation and foot printing
B. Northem analysis and RT-PCR
C. Primer extension and Sl nuclease mapping
D. Cloning of 5' UTRs and DNA Sequencing
6.Which one of the following is a Ml3 based vector
A.pBR322
B.pT7blue
C. pUC 18
D. Cosmid
7.DNA fragment size suitable to cloning in a l" replacement vector
A. 30 kb
B.35 kb
c. 18 kb
D. 53 kb
8.In a yeast two hybrid assay if two test proteins (bait and prey) are interacting with each other, then
A.LacZ reporter gene will be strongly repressed
B.LacZ reporter gene will be expressed
C.LacZ reporter gene expression is repressed by LacI
D.LacZ reporter gene expression is unaffected
9.RNA is susceptible to alkaline hydrolysis, but not DNA because
A. RNA is single stranded and nitrogenous bases are exposed to alkali
B.RNA can form intra base-pairing, but not DNA
C.RNA has 2'-OH group
D. RNA is smaller than DNA
10. Which one of the following has high gene density
A. Human
B.Arabidopsis
C.Drosophila
D. E.coli
I 1. Processivity of DNA polymerase is enhanced by
A. DNA helicase
B. Sliding clamp
C. Topoisomerase
D. RNA helicase
12. Which form of the following ptasmid DNA migrates faster during agarose gel electrophoresis
A. highly super-coiled plasmid DNA
B. relaxed plasmid DNA
C. linear plasmid DNA
D. nicked circular plasmid DNA
13. Which one of the following E.coli sigma subunit is required for transcription of heat inducible genes
A. Sigma 70
B. Sigma 32
C. Sigma54
D. Sigma28
14. Which one of the following is NOT a state function?
A. lntemal energy
B. Heat
C. Enthalpy
D. Entropy
15. Number of vibrational degrees of freedom for ethylene molecule is
A. 18
B. l5
c.12
D.l I
16. Symmetry elements of ammonia molecule contain
A. Cs-axis of symmetry and one centre of symmetry
B. Cl-axis of symmetry and three planes of symmetry
C. G-axis of symmetry and one centre of symmetry
D. Cz-axis of symmetry and two planes of symmetry
17. Volume of a balloon filled with an ideal gas is 5 L, at 1 atm and 25 oC; what will be the volume of the balloon if temperature increases to 35oC?
A.3.57 L
8.4.84 L
c.5.17 t.
D,7.0 L
18. Which of the following sequence is conect, if the molecules are arranged according to their bond energies?
A. Hz> F2> Cl2> Br2
B. Hz> Cl2>F2> Br2
C. Fz> Clz> Brz>Hz
D. Clz> F2> Br2>H2
19. Addition of an inhibitor does not change K^lV**value, if the mechanism of inhibition rs
A. Competitive
B. Uncompetitive
C. Noncompetitive
D. All the above
20. Generally, in a fluorescent experiment, moving the excitation wavelength to higher wavelength,
A. does not affect the emission wavelength
B. blue shifts emission wavelength
C. red shifts emission wavelength
D. depends on the solvent and temperature
21. The chloroplast genome of higher plants contain
A. Twelve genes
B. Hundred and twenty genes
C. Twelve hundred genes
D. Twelve thousand genes
22. The metabolite that causes internal bleeding in mammals
A. Stilbenes
B. Coumarins
C. Ferulic acid
D. Chalcones
23. Denaturation of aprotein involves
A. Partial loss of amino acid sequence
B. Loss of s-helices
C. Loss of p-sheets
D. Loss of 3-D shape and catalytic activity
24. Aparticle moves with a constant velocity parallel to the X-axis. Its angular momentum with respect to the origin,
A. Is zero
B. Remains constant
C. Goes on decreasing
D. Goes on increasing
25.The secondary pacemaker of the heart is known as the:
A. AV node
B. SA node
C. Purkinje Bundle
D. None of the above
1. Which one of the following techniques is used for studying genome wide differential gene expression
A.Western blotting
B. Northern blotting
C. DNA microarray
D. Pyro-sequencing of genomes
2.Which of the following BLAS'f programs is used for protein query against a translated nucleotide sequence database
A. Blastp
B. Blastn
C. tblastx
D. Tblastn
3.Which of the following amino acids is least mutable according to the PAM scoring matrix?
A. Alanine
B. Glutamine
C. Methionine
D. Cysteine
4.If the ratio A26sl A2ss nm of a DNA solution is 1 .4, it indicates contamination of DNA
preparation with
A. Carbohydrate
B. RNA
C. Protein
D. Salt
5.The methods used for locating transcription start site are
A. Gel retardation and foot printing
B. Northem analysis and RT-PCR
C. Primer extension and Sl nuclease mapping
D. Cloning of 5' UTRs and DNA Sequencing
6.Which one of the following is a Ml3 based vector
A.pBR322
B.pT7blue
C. pUC 18
D. Cosmid
7.DNA fragment size suitable to cloning in a l" replacement vector
A. 30 kb
B.35 kb
c. 18 kb
D. 53 kb
8.In a yeast two hybrid assay if two test proteins (bait and prey) are interacting with each other, then
A.LacZ reporter gene will be strongly repressed
B.LacZ reporter gene will be expressed
C.LacZ reporter gene expression is repressed by LacI
D.LacZ reporter gene expression is unaffected
9.RNA is susceptible to alkaline hydrolysis, but not DNA because
A. RNA is single stranded and nitrogenous bases are exposed to alkali
B.RNA can form intra base-pairing, but not DNA
C.RNA has 2'-OH group
D. RNA is smaller than DNA
10. Which one of the following has high gene density
A. Human
B.Arabidopsis
C.Drosophila
D. E.coli
I 1. Processivity of DNA polymerase is enhanced by
A. DNA helicase
B. Sliding clamp
C. Topoisomerase
D. RNA helicase
12. Which form of the following ptasmid DNA migrates faster during agarose gel electrophoresis
A. highly super-coiled plasmid DNA
B. relaxed plasmid DNA
C. linear plasmid DNA
D. nicked circular plasmid DNA
13. Which one of the following E.coli sigma subunit is required for transcription of heat inducible genes
A. Sigma 70
B. Sigma 32
C. Sigma54
D. Sigma28
14. Which one of the following is NOT a state function?
A. lntemal energy
B. Heat
C. Enthalpy
D. Entropy
15. Number of vibrational degrees of freedom for ethylene molecule is
A. 18
B. l5
c.12
D.l I
16. Symmetry elements of ammonia molecule contain
A. Cs-axis of symmetry and one centre of symmetry
B. Cl-axis of symmetry and three planes of symmetry
C. G-axis of symmetry and one centre of symmetry
D. Cz-axis of symmetry and two planes of symmetry
17. Volume of a balloon filled with an ideal gas is 5 L, at 1 atm and 25 oC; what will be the volume of the balloon if temperature increases to 35oC?
A.3.57 L
8.4.84 L
c.5.17 t.
D,7.0 L
18. Which of the following sequence is conect, if the molecules are arranged according to their bond energies?
A. Hz> F2> Cl2> Br2
B. Hz> Cl2>F2> Br2
C. Fz> Clz> Brz>Hz
D. Clz> F2> Br2>H2
19. Addition of an inhibitor does not change K^lV**value, if the mechanism of inhibition rs
A. Competitive
B. Uncompetitive
C. Noncompetitive
D. All the above
20. Generally, in a fluorescent experiment, moving the excitation wavelength to higher wavelength,
A. does not affect the emission wavelength
B. blue shifts emission wavelength
C. red shifts emission wavelength
D. depends on the solvent and temperature
21. The chloroplast genome of higher plants contain
A. Twelve genes
B. Hundred and twenty genes
C. Twelve hundred genes
D. Twelve thousand genes
22. The metabolite that causes internal bleeding in mammals
A. Stilbenes
B. Coumarins
C. Ferulic acid
D. Chalcones
23. Denaturation of aprotein involves
A. Partial loss of amino acid sequence
B. Loss of s-helices
C. Loss of p-sheets
D. Loss of 3-D shape and catalytic activity
24. Aparticle moves with a constant velocity parallel to the X-axis. Its angular momentum with respect to the origin,
A. Is zero
B. Remains constant
C. Goes on decreasing
D. Goes on increasing
25.The secondary pacemaker of the heart is known as the:
A. AV node
B. SA node
C. Purkinje Bundle
D. None of the above
Wednesday, 18 March 2015
University of Hyderabad 2012 Phd Plantsciences PART B PAPER
PART -B
26. The largest family in the flowering plants is
A. Graminae
B. Compositae
C.Orchidaceae
D. Leguminosae
27 . What is "Phosgeneo' ?
A. It is a type of pseudo gene which is predicted by a software
B. It is type a gene which is rich in phosphoric acids
C. It is colorless poisonous gas
D. It is a type antibiotic which inhibits bacterial gene expression
28. The practice of reducing or eliminating tillage operations and leaving crop residues on the soil to prevent erosion.
A.Green manure
B. Biofertilizer
C. Conservation tillage
D. Green revolution
29. One of enzymes highly sensitive to and is inhibited by oxygen is
A. Rubisco
B. cytochrome oxidase
C. polyphenol oxidase
D. nitrogenase
30. Fraction I protein in plants is also known as
A. bovine serum albumin
B. seed protein
C. protein fraction
D. Rubisco
31. The following gives rise to the endomembrane system in a cell
A. Plasma membrane
B. Mitochondria
C. Endoplasmic Reticulum
D. Golgi Apparatus
32' Recently, which company's GM-Brinjal has not been given permission for commercial cultivation
A. Nuzvid seeds
B. Advanta India
C. Raas Seeds
D. Mahyco .phytoene,
33. one of the following statements related to is wrong
A. Belongs to the class of terpenoids
B. Precursor of Carotenoids
c. synthesised from two molecules of geranylgeranyl diphophate
D. Precursor of phosph o-enol-pyruvate
34. Fertilization in Pteridophytes is
A. Apogamy
B. Syngamy
C. Apospory
D. Siphanogamy
35. Find out the mislpatgh for the following enzymes and their products
A. Pyruvate dehydrogenase -Acetyl CoA, CO2, NADH
B. Pynrvate decarboxylase -Acetaldehyde, COz
C. Pynrvate-formate lyase -Acetyl CoA, Formate, NAD
D. Pynrvate oxidase -Acetyl pO+, COz, FADH
36. Cinchona plant belongs to
A. Apocyanaceae
B. Asteraceae
C. Rubiaceae
D. Lamiaceae
37. Biological modification that allows species to better exist in a specificenvironment for short term.
A. Ecotype
B. Ecad
C. Acclimation
D. Adaptation
38.In tetrad analysis, non-parental ditype asci can result from:
A. single crossovers between linked genes
B. single crossovers between a gene and a centromer
C. double crossovers between linked genes
D. double crossovers between a gene and a centromere
39. A mixture of several herbicides that is considered to be a carcinogen because it is
contaminated with dioxin.
A. Agent oftmge
B. Multi-Weedicide
C. Pesticide
D. Chlorofluorocarbons CFCs
40. which one of the following statements about the epidermis is f?lse?
A. On the plant body, only leaves are covered by a cuticle
B. The vast majority of epidermal cells are parenchymatous
C. Guard cells are normally the only epidermal cells to have well developed chloroplasts
D. subsidiary cells are associated with guard cells in many plant groups
41. An organism with two different alleles is called
A. homozygous for that trait
B. homologous for the allele
C. heterozygous for that trait
D. heterologous for the allele
42.Inhigher plants, cytochrome c is typically located in
A. plasma membrane
B. chloroplasts
C. mitochondria
D. cytoplasm
43.In a cross involving polygenic inheritance, only lnD24 of the offspring in F2 generation were as extreme as one of the parents. How many gene pairs are involved?
A.3
8.4
c.5
D.6
44' Designated sites that have been abandoned or underused because of real or perceived environmental contaimination/pollution are called as
A. Green field
B. Brown fied
C. White field
D. Red field
45' A column chromatography method that makes use of a specific ligand moleculethat is attached to an insoluble matrix, and is .upuUi. of uirrAirrg 6 tt. ,notrrutebeing purified is known as
A. Ion exchange chromatography
B. Partition chromatography
C. Gel filtration chromatograph
D. Affinity chromato graphy
46' Match the following diseases in group A with their causative agent in group B.
Groun A
K. Histoplasmosis
L. Encephalitis
M. Cysticercosis
N. Leishmaniasis
A. K-4; L-l; M-2; N-5
B. K-2;L-4; M-1; N-5
C. K-3; L-1; M-2; N-5
D. K-3; L-t; M-5; N-2
Group B
1. Virus
2. Tapeworm
3. Fungi
4. Bacteria
5. Protozoa
47.The alleles A, B and c are on the same maternal cbromosome and a, b and c are on the same patemal cluomosome. The gnly way that hetero rygotewill produce a gamete with alleles a, b and C is through
A. nondisjunction
B. the laws of segregation
C. the law of independent assortment
D. crossing over
48. When leaves become water-stressed, they wilt, because.......
A. increased ABA levels have caused the stomata to close
B. phloem transport has ceased leading to increased sugar levels
C. their mesophyll cells are no longer turgid
D. their guard cells are no longer turgid
49. Restoring a natural area by the addition of living organisms e.g., plants or bacteria.
A. Bioremediation
B. Phytoremediation
C. Phycoremediation
D. Rhizoremediation
50. Husk of coconut is made of:
A. Schlerenchyma
B. Parenchyma
C. Collenchyma
D. Prosenchyma
51. Identifu the mismatch
A. Selective medium * Bismuth sulfite agar
B. Differential medium -MacConkey agar
C. Enrichment medium -Lysine iron agar
D. Characteristic medium -Sulfide, indole, motility SIM medium
52, Apart of the biosphere that absorbs more carbon dioxide than it releases; e.g.,oceans and rain forests.
A. Carbon sink
B. Acid mine
C. Peat bog
D. Alkaline soil
53. In Arabidopsis, expression of one of the genes was suppressed by RNA interference technology. The following technique gan not be used for testing the levels of that specific gene product in the mutant series:
A. Northern analyses with total RNA of mutants
B. Southern analyses with genomic DNA of mutants
C. RT-PCR analyses with oDNA prepared with total RNA of mutants
D. Wetem analyses with total protein isolated from mutants
54. Which of the following is a symptom of magnesium deficiency?
A. Yellowing of younger leaves' prior to yellowing of older leaves.
B. Enhanced plant growth, since magnesium is toxic to plants.
C. Chlorosis
D. decreased transpiration
55. One of the following member do not belong to the family Enterobacteriaceae
A. Proteus
B. Yersinia
C. Citrobacter
D. Vibrio
56. Fatty acyl CoA in cytoplasm is transported to outer mitochondrial membrane for Beta-oxidation as
A. Glycolipids
B. Acyl carnitine
C. Acteyl CoA
D. Malonyl-CoA
57. Identifu the mismatch
A, Bacitracin -inhibits cell wall synthesis
B. Fusidic acid -Binds to EF-G and blocks translocation
C. Dapsone -Interferes with folic acid synthesis
D. Polymyxin B -Inhibits the synthesis of the mycolic acid "cord factor"
58. Ammonia oxidation to nitrate depends on the following two bacteria
A. Nitr o s omonas -Nitro s o spir a
B. Azo spir illum-P s eudomo nqs
C. Nitrobacter-Nitr o c o ccus
D. N itr o s o spir a-Nitr o c o c cus
59. Seed dormancy inhibited mechanically by causing seed coat injury is called as:
A. Stratification
B. Scarification
C. Vernalization
D. Bahar treatment
60. Eukaryotic and multicellular body pattems are not found in
A. Monera
B. Protista
C. Fungi
D. Animalia
61. Gene-for-gene hypothesis was given by:
A. Gregor Johann Mendel
B. Darwin
C. Robert Koch
D. Harold Henry Flor
62. Presently, there are lot more variants of 'Green {luorescent proteins' are available and are used in in vivo imaging, However, the basic protein is isolated from
A. Gloriosa superba
B. Aequorea Victoria
C. Thermus aquaticus
D. Microsporilla gramina
63. Tabtoxin is toxic to plant cells because it inactivates the enzyme glutamine synthetase, which leads to depletion of glutamine levels, and accumulation of
A. Aspartate
B. Ammonia
C. Acetic acid
D. Benzoic acid
64. The two-carbon compound which acts as substrate for photorespiration is
A. glycine
B. glycolate
C. acetyl CoA
D. malate
65. NADP'malic enzyme is located in the following compartment of bundle sheath cells
A. chloroplast
B. mitochondria
C. peroxisome
D. cytoplasm
66. Mutations that involve a change in single nucleotide resulting in a codon that code for a different aminoacid are
A. missense mutation
B. nonsense mutation
C. frameshift mutations
D. transversions
67. Femedoxin can be called as a
A. iron-sulphur protein
B. porphyrin protein
C. sulphur-magnesium protein
D. non-haem protein
68. One of the following proteins of virulence region on the Ti Plasmid is autokinase
A. VirA
B. VirD4
C. VirBS
D. VirH
69. A ferv fungi accumulate this metabolite during maturation of the aspersorium, which allows drawaing of more water to build hydrostatic pressrue.
A. Galaturonic acid
B. Strigol
C. Glycerol
D. Cutin
70. One of the following Scientists is associated with the development of gene gun
A. Hargobind Khorana
B. D. Baltimore
C. Eric Reeves
D. J.C. Sanford
71. Golden age of Gymnospenns
A, Paleozoic era
B. Mesozoic era
C. Coenozoic era
D. Devonion period
72. An organism that lives part of its life as a parasite on another organism and the other part as a saprophyte is called
A. Hemibiotoph
B. Biotroph
C. Halophyte
D Lithophyte
73. Bulbosum system employed in barley for production of haploids involved all of the following ex.gent:
A. Wide hybridization
B.Invitro immature zygotic embryo culture
C. Elimination of the chromosomes of vulgare
D. Elimination of the chromosomes of bulbosum
74. Phaseollin is a very important compound that has great significance in deciding the fate of the host-pathogen interactions. This compound is
A. Produced by the pathogen
B. Produced by the host
C. Chemical fungicide
D. Biological weedicide
75. The entry of most of the bacterial pathogens into the plants occurs through the
following.
A. Direct penetration of the cell walls
B. Enter tfuough the wounds made by vectors
C. Physical contact with the infbcted plant
D. Natural openings of the plants
26. The largest family in the flowering plants is
A. Graminae
B. Compositae
C.Orchidaceae
D. Leguminosae
27 . What is "Phosgeneo' ?
A. It is a type of pseudo gene which is predicted by a software
B. It is type a gene which is rich in phosphoric acids
C. It is colorless poisonous gas
D. It is a type antibiotic which inhibits bacterial gene expression
28. The practice of reducing or eliminating tillage operations and leaving crop residues on the soil to prevent erosion.
A.Green manure
B. Biofertilizer
C. Conservation tillage
D. Green revolution
29. One of enzymes highly sensitive to and is inhibited by oxygen is
A. Rubisco
B. cytochrome oxidase
C. polyphenol oxidase
D. nitrogenase
30. Fraction I protein in plants is also known as
A. bovine serum albumin
B. seed protein
C. protein fraction
D. Rubisco
31. The following gives rise to the endomembrane system in a cell
A. Plasma membrane
B. Mitochondria
C. Endoplasmic Reticulum
D. Golgi Apparatus
32' Recently, which company's GM-Brinjal has not been given permission for commercial cultivation
A. Nuzvid seeds
B. Advanta India
C. Raas Seeds
D. Mahyco .phytoene,
33. one of the following statements related to is wrong
A. Belongs to the class of terpenoids
B. Precursor of Carotenoids
c. synthesised from two molecules of geranylgeranyl diphophate
D. Precursor of phosph o-enol-pyruvate
34. Fertilization in Pteridophytes is
A. Apogamy
B. Syngamy
C. Apospory
D. Siphanogamy
35. Find out the mislpatgh for the following enzymes and their products
A. Pyruvate dehydrogenase -Acetyl CoA, CO2, NADH
B. Pynrvate decarboxylase -Acetaldehyde, COz
C. Pynrvate-formate lyase -Acetyl CoA, Formate, NAD
D. Pynrvate oxidase -Acetyl pO+, COz, FADH
36. Cinchona plant belongs to
A. Apocyanaceae
B. Asteraceae
C. Rubiaceae
D. Lamiaceae
37. Biological modification that allows species to better exist in a specificenvironment for short term.
A. Ecotype
B. Ecad
C. Acclimation
D. Adaptation
38.In tetrad analysis, non-parental ditype asci can result from:
A. single crossovers between linked genes
B. single crossovers between a gene and a centromer
C. double crossovers between linked genes
D. double crossovers between a gene and a centromere
39. A mixture of several herbicides that is considered to be a carcinogen because it is
contaminated with dioxin.
A. Agent oftmge
B. Multi-Weedicide
C. Pesticide
D. Chlorofluorocarbons CFCs
40. which one of the following statements about the epidermis is f?lse?
A. On the plant body, only leaves are covered by a cuticle
B. The vast majority of epidermal cells are parenchymatous
C. Guard cells are normally the only epidermal cells to have well developed chloroplasts
D. subsidiary cells are associated with guard cells in many plant groups
41. An organism with two different alleles is called
A. homozygous for that trait
B. homologous for the allele
C. heterozygous for that trait
D. heterologous for the allele
42.Inhigher plants, cytochrome c is typically located in
A. plasma membrane
B. chloroplasts
C. mitochondria
D. cytoplasm
43.In a cross involving polygenic inheritance, only lnD24 of the offspring in F2 generation were as extreme as one of the parents. How many gene pairs are involved?
A.3
8.4
c.5
D.6
44' Designated sites that have been abandoned or underused because of real or perceived environmental contaimination/pollution are called as
A. Green field
B. Brown fied
C. White field
D. Red field
45' A column chromatography method that makes use of a specific ligand moleculethat is attached to an insoluble matrix, and is .upuUi. of uirrAirrg 6 tt. ,notrrutebeing purified is known as
A. Ion exchange chromatography
B. Partition chromatography
C. Gel filtration chromatograph
D. Affinity chromato graphy
46' Match the following diseases in group A with their causative agent in group B.
Groun A
K. Histoplasmosis
L. Encephalitis
M. Cysticercosis
N. Leishmaniasis
A. K-4; L-l; M-2; N-5
B. K-2;L-4; M-1; N-5
C. K-3; L-1; M-2; N-5
D. K-3; L-t; M-5; N-2
Group B
1. Virus
2. Tapeworm
3. Fungi
4. Bacteria
5. Protozoa
47.The alleles A, B and c are on the same maternal cbromosome and a, b and c are on the same patemal cluomosome. The gnly way that hetero rygotewill produce a gamete with alleles a, b and C is through
A. nondisjunction
B. the laws of segregation
C. the law of independent assortment
D. crossing over
48. When leaves become water-stressed, they wilt, because.......
A. increased ABA levels have caused the stomata to close
B. phloem transport has ceased leading to increased sugar levels
C. their mesophyll cells are no longer turgid
D. their guard cells are no longer turgid
49. Restoring a natural area by the addition of living organisms e.g., plants or bacteria.
A. Bioremediation
B. Phytoremediation
C. Phycoremediation
D. Rhizoremediation
50. Husk of coconut is made of:
A. Schlerenchyma
B. Parenchyma
C. Collenchyma
D. Prosenchyma
51. Identifu the mismatch
A. Selective medium * Bismuth sulfite agar
B. Differential medium -MacConkey agar
C. Enrichment medium -Lysine iron agar
D. Characteristic medium -Sulfide, indole, motility SIM medium
52, Apart of the biosphere that absorbs more carbon dioxide than it releases; e.g.,oceans and rain forests.
A. Carbon sink
B. Acid mine
C. Peat bog
D. Alkaline soil
53. In Arabidopsis, expression of one of the genes was suppressed by RNA interference technology. The following technique gan not be used for testing the levels of that specific gene product in the mutant series:
A. Northern analyses with total RNA of mutants
B. Southern analyses with genomic DNA of mutants
C. RT-PCR analyses with oDNA prepared with total RNA of mutants
D. Wetem analyses with total protein isolated from mutants
54. Which of the following is a symptom of magnesium deficiency?
A. Yellowing of younger leaves' prior to yellowing of older leaves.
B. Enhanced plant growth, since magnesium is toxic to plants.
C. Chlorosis
D. decreased transpiration
55. One of the following member do not belong to the family Enterobacteriaceae
A. Proteus
B. Yersinia
C. Citrobacter
D. Vibrio
56. Fatty acyl CoA in cytoplasm is transported to outer mitochondrial membrane for Beta-oxidation as
A. Glycolipids
B. Acyl carnitine
C. Acteyl CoA
D. Malonyl-CoA
57. Identifu the mismatch
A, Bacitracin -inhibits cell wall synthesis
B. Fusidic acid -Binds to EF-G and blocks translocation
C. Dapsone -Interferes with folic acid synthesis
D. Polymyxin B -Inhibits the synthesis of the mycolic acid "cord factor"
58. Ammonia oxidation to nitrate depends on the following two bacteria
A. Nitr o s omonas -Nitro s o spir a
B. Azo spir illum-P s eudomo nqs
C. Nitrobacter-Nitr o c o ccus
D. N itr o s o spir a-Nitr o c o c cus
59. Seed dormancy inhibited mechanically by causing seed coat injury is called as:
A. Stratification
B. Scarification
C. Vernalization
D. Bahar treatment
60. Eukaryotic and multicellular body pattems are not found in
A. Monera
B. Protista
C. Fungi
D. Animalia
61. Gene-for-gene hypothesis was given by:
A. Gregor Johann Mendel
B. Darwin
C. Robert Koch
D. Harold Henry Flor
62. Presently, there are lot more variants of 'Green {luorescent proteins' are available and are used in in vivo imaging, However, the basic protein is isolated from
A. Gloriosa superba
B. Aequorea Victoria
C. Thermus aquaticus
D. Microsporilla gramina
63. Tabtoxin is toxic to plant cells because it inactivates the enzyme glutamine synthetase, which leads to depletion of glutamine levels, and accumulation of
A. Aspartate
B. Ammonia
C. Acetic acid
D. Benzoic acid
64. The two-carbon compound which acts as substrate for photorespiration is
A. glycine
B. glycolate
C. acetyl CoA
D. malate
65. NADP'malic enzyme is located in the following compartment of bundle sheath cells
A. chloroplast
B. mitochondria
C. peroxisome
D. cytoplasm
66. Mutations that involve a change in single nucleotide resulting in a codon that code for a different aminoacid are
A. missense mutation
B. nonsense mutation
C. frameshift mutations
D. transversions
67. Femedoxin can be called as a
A. iron-sulphur protein
B. porphyrin protein
C. sulphur-magnesium protein
D. non-haem protein
68. One of the following proteins of virulence region on the Ti Plasmid is autokinase
A. VirA
B. VirD4
C. VirBS
D. VirH
69. A ferv fungi accumulate this metabolite during maturation of the aspersorium, which allows drawaing of more water to build hydrostatic pressrue.
A. Galaturonic acid
B. Strigol
C. Glycerol
D. Cutin
70. One of the following Scientists is associated with the development of gene gun
A. Hargobind Khorana
B. D. Baltimore
C. Eric Reeves
D. J.C. Sanford
71. Golden age of Gymnospenns
A, Paleozoic era
B. Mesozoic era
C. Coenozoic era
D. Devonion period
72. An organism that lives part of its life as a parasite on another organism and the other part as a saprophyte is called
A. Hemibiotoph
B. Biotroph
C. Halophyte
D Lithophyte
73. Bulbosum system employed in barley for production of haploids involved all of the following ex.gent:
A. Wide hybridization
B.Invitro immature zygotic embryo culture
C. Elimination of the chromosomes of vulgare
D. Elimination of the chromosomes of bulbosum
74. Phaseollin is a very important compound that has great significance in deciding the fate of the host-pathogen interactions. This compound is
A. Produced by the pathogen
B. Produced by the host
C. Chemical fungicide
D. Biological weedicide
75. The entry of most of the bacterial pathogens into the plants occurs through the
following.
A. Direct penetration of the cell walls
B. Enter tfuough the wounds made by vectors
C. Physical contact with the infbcted plant
D. Natural openings of the plants
Tuesday, 17 March 2015
University of Hyderabad 2012 Ph.D Plant Sciences PART A
1. An increase in some phenomenon at a constant rate over a specified time period.
A. Geometic growth
B. Exponential
C. Arithmetic growth
D. Nonlinear
2. Which of the following plant hormones is incorrectly paired with its function?
A. cytokinin -promotes senescence
B. gibberellin -stimulates seed germination
C. abscisic acid -promotes seed and bud dormancy
D. ethylene -promotes fruit ripening
3. A mixture containing the following compounds is passed through a column in a gel filtration chromatography, which excludes all protein of MW 100,000 and higher: protein A MW = 30,000, protein B MW = 200,000, protein C MW : 90,000, protein D MW : 50,000, protein E MW: 250,000. What will be the order of elution of these proteins ?
A. Protein E, Protein B, Protein C, Protein D, Protein A
B. Protein E + Protein B, Protein C, Protein D, Protein A
C. Protein A, Protein D, Protein C, Protein B, Protein E
D. Protein E + Protein B, Protein A, Protein D, Protein C
4. Verticillaster influorescence is a feature of
A. Malvaceae
B. Liliaceae
C. Labiatae
D. Cruciferae
5. Which of the following regarding epigenetic inheritance is false?
A. It does not involve changes in DNA sequence
B. It involves functionally relevant modifications to the genome such as histone modification
C. Epigenetic changes are not preserved when cells divide
D. Epgenetic inheritance can be reset during gametogenesis
6. If you could connect and active xyiem vessel from a shoot to an active phloem sieve-tube member from a leaf using a r'micropipe," which way would the solution flow between the two?
A. The solution wouid flow from xylem to phloem.
B. The solution would flow from phloem to xylem.
C. The solution would flow back and forth from one to anotler.
D.The solution would not flow between the two.
7.If the map distance between genes A and B is 5 map units and the map distance between B and c is 15 map units, what is the map distance between A and C?
A. 5 map units
B. 10 map units
C. 20 map units
D. Either 10 map units or 20 map units depending on the order of genes
8.If a cell with a solute potential of -0.2 MPa and a pressure potential of 0.4 MPa is placed in a chamber filled with pure water that is pressurized with 0.5 MP4 what will happen?
A. Water will flow out of the cell.
B. Water will flow into the cell.
C The cell will be crushed.
D. The cell will explode.
9, The firngus that causes southern com leaf blight is known to produce this host specific toxin.
A. HT toxin
B. HV toxin
C. HC toxin
D. HS toxin
10. Primitive bryophytes are called
A. Club mosses
B. Horse tails
C. Liver worts
D. Ferns
11. The bioluminescent dinoflagellates are
A. Noctiluca and Gonyaulax
B. Glmnodinium and Cerastium
C. Dinobryon arrd Distephanus
D. Pinnularta nd Acetabularia
12. Which one of the following is living but non-nucleated:
A. Sieve tube
B. Companion cells
C. Phloem fibre
D. Phloem parenchyma
13. uranyl acetate is used as a negative strain in this technique
A. Confocal microscopy
B. Epifluorescence
C Electron microscopy
D. Compound microscope
14. Protoplasts can be made from the plant cells using these enzyme combinations
A. Cellulase and Pectinase
B. Replicase and Ligase
C. Protease and Chitinase
D. DNAse and RNase
15. A large ecosytem that has distinct climate, geology, and organisms; e.g., desert,tunfta, grassland, savilm4 woodland, coniferous forest, temperate deciduous forest, and tropical rain forest.
A. Ecotone
B. Microcosm
C. Global ecosystem
D. Biome
16. Roger Beachy is known for this landmark discovery in plant pathology.
A. Transgenic plants resistant to insects
B. Transgenic plants resistant to fungal pathogens
C. Transgenic plants resistant to bacterial puthogeos
D. ltansgenic plants resistant to plant viruses
17. During calvin cycle, the last enzyme involved in RuBp regeneration is
A. phosphoribulokinase
B. Rubisco
(. phosphopentoepimerase
D. phosphopentoseisomerase
18..A local movement that began in India in the early l9g0s and is opposed to govemmental deforestation programs.
A. Green movement
B. Afforestation
C. Heat Island
D. Chipko movement
19. Salicylic acid is known to induce systemic aequirecl resistance SAR in plants,Identi$ one among the following, which is also known to induce SAR.
A. Succinic acid
B. Giuconic acid
C. 2,6-dichloroisonicotinic acid
D. Chorisrnic acid
20, A major route for biosynthesis of secondary products in plants is
A. C+ pathway
B. Shikimate pathway
C. CAM pathway
D. GS-GOGAT system
21. Number of 'High Energy P' bonds are required to translat e a279 aa protein
A.279
B.558
c.837
D. 1116
22. One of the following proteins is positioned as the tip of the pilus formed between the Agrobacterium and plant cell
A. VirBl
B. VirBl l
C. VirB5
D. VirA
23. A reaction medium of 500 ml containing2} mM Tris-HCl buffer pH 7.5, 0.5 mM MgCl2 and 0.05% NaN3 has to be prepared using the stock solutions of I M Tris HCI pH 7.5; 100 mM MgCl2 and 5% NaN3 solution. The volumes of stocks should be mixed as
A. 10 ml of Tris-HCl, 5 ml of MgCD and 2.5 ml of NaN3 in482.5 ml of ddH2O
B. 50 ml of Tris-HCl, 5 ml of MgCl2 and2.5 ml of Na]'{3 in442.5 ml of ddH2O
C. 10 ml of Tris-HCl, 2.5 ml of MgCl2 and 5 ml ofNaN3 in 482.5 ml of ddH2O
D. 50 ml of Tris-HCI,2.5 ml of MgCl2 and 5 ml of NaN3 in442.5 ml of ddH2O
24. Which of the following is a marker gene in chloroplast transformation studies
A. Phosphomannose isomerase
B. Xyiose isomerase
C. Chloramphenicol acetyl transferase
D. Aminoglycoside adenyl transferase
25. Primary amino sequence of a protein from five plant species is given to you.
Which of the following tools would you use to know the homology between the
sequences?
A. BLAST
B. TargetP
C. CLUSTALW
D. Primer Express
A. Geometic growth
B. Exponential
C. Arithmetic growth
D. Nonlinear
2. Which of the following plant hormones is incorrectly paired with its function?
A. cytokinin -promotes senescence
B. gibberellin -stimulates seed germination
C. abscisic acid -promotes seed and bud dormancy
D. ethylene -promotes fruit ripening
3. A mixture containing the following compounds is passed through a column in a gel filtration chromatography, which excludes all protein of MW 100,000 and higher: protein A MW = 30,000, protein B MW = 200,000, protein C MW : 90,000, protein D MW : 50,000, protein E MW: 250,000. What will be the order of elution of these proteins ?
A. Protein E, Protein B, Protein C, Protein D, Protein A
B. Protein E + Protein B, Protein C, Protein D, Protein A
C. Protein A, Protein D, Protein C, Protein B, Protein E
D. Protein E + Protein B, Protein A, Protein D, Protein C
4. Verticillaster influorescence is a feature of
A. Malvaceae
B. Liliaceae
C. Labiatae
D. Cruciferae
5. Which of the following regarding epigenetic inheritance is false?
A. It does not involve changes in DNA sequence
B. It involves functionally relevant modifications to the genome such as histone modification
C. Epigenetic changes are not preserved when cells divide
D. Epgenetic inheritance can be reset during gametogenesis
6. If you could connect and active xyiem vessel from a shoot to an active phloem sieve-tube member from a leaf using a r'micropipe," which way would the solution flow between the two?
A. The solution wouid flow from xylem to phloem.
B. The solution would flow from phloem to xylem.
C. The solution would flow back and forth from one to anotler.
D.The solution would not flow between the two.
7.If the map distance between genes A and B is 5 map units and the map distance between B and c is 15 map units, what is the map distance between A and C?
A. 5 map units
B. 10 map units
C. 20 map units
D. Either 10 map units or 20 map units depending on the order of genes
8.If a cell with a solute potential of -0.2 MPa and a pressure potential of 0.4 MPa is placed in a chamber filled with pure water that is pressurized with 0.5 MP4 what will happen?
A. Water will flow out of the cell.
B. Water will flow into the cell.
C The cell will be crushed.
D. The cell will explode.
9, The firngus that causes southern com leaf blight is known to produce this host specific toxin.
A. HT toxin
B. HV toxin
C. HC toxin
D. HS toxin
10. Primitive bryophytes are called
A. Club mosses
B. Horse tails
C. Liver worts
D. Ferns
11. The bioluminescent dinoflagellates are
A. Noctiluca and Gonyaulax
B. Glmnodinium and Cerastium
C. Dinobryon arrd Distephanus
D. Pinnularta nd Acetabularia
12. Which one of the following is living but non-nucleated:
A. Sieve tube
B. Companion cells
C. Phloem fibre
D. Phloem parenchyma
13. uranyl acetate is used as a negative strain in this technique
A. Confocal microscopy
B. Epifluorescence
C Electron microscopy
D. Compound microscope
14. Protoplasts can be made from the plant cells using these enzyme combinations
A. Cellulase and Pectinase
B. Replicase and Ligase
C. Protease and Chitinase
D. DNAse and RNase
15. A large ecosytem that has distinct climate, geology, and organisms; e.g., desert,tunfta, grassland, savilm4 woodland, coniferous forest, temperate deciduous forest, and tropical rain forest.
A. Ecotone
B. Microcosm
C. Global ecosystem
D. Biome
16. Roger Beachy is known for this landmark discovery in plant pathology.
A. Transgenic plants resistant to insects
B. Transgenic plants resistant to fungal pathogens
C. Transgenic plants resistant to bacterial puthogeos
D. ltansgenic plants resistant to plant viruses
17. During calvin cycle, the last enzyme involved in RuBp regeneration is
A. phosphoribulokinase
B. Rubisco
(. phosphopentoepimerase
D. phosphopentoseisomerase
18..A local movement that began in India in the early l9g0s and is opposed to govemmental deforestation programs.
A. Green movement
B. Afforestation
C. Heat Island
D. Chipko movement
19. Salicylic acid is known to induce systemic aequirecl resistance SAR in plants,Identi$ one among the following, which is also known to induce SAR.
A. Succinic acid
B. Giuconic acid
C. 2,6-dichloroisonicotinic acid
D. Chorisrnic acid
20, A major route for biosynthesis of secondary products in plants is
A. C+ pathway
B. Shikimate pathway
C. CAM pathway
D. GS-GOGAT system
21. Number of 'High Energy P' bonds are required to translat e a279 aa protein
A.279
B.558
c.837
D. 1116
22. One of the following proteins is positioned as the tip of the pilus formed between the Agrobacterium and plant cell
A. VirBl
B. VirBl l
C. VirB5
D. VirA
23. A reaction medium of 500 ml containing2} mM Tris-HCl buffer pH 7.5, 0.5 mM MgCl2 and 0.05% NaN3 has to be prepared using the stock solutions of I M Tris HCI pH 7.5; 100 mM MgCl2 and 5% NaN3 solution. The volumes of stocks should be mixed as
A. 10 ml of Tris-HCl, 5 ml of MgCD and 2.5 ml of NaN3 in482.5 ml of ddH2O
B. 50 ml of Tris-HCl, 5 ml of MgCl2 and2.5 ml of Na]'{3 in442.5 ml of ddH2O
C. 10 ml of Tris-HCl, 2.5 ml of MgCl2 and 5 ml ofNaN3 in 482.5 ml of ddH2O
D. 50 ml of Tris-HCI,2.5 ml of MgCl2 and 5 ml of NaN3 in442.5 ml of ddH2O
24. Which of the following is a marker gene in chloroplast transformation studies
A. Phosphomannose isomerase
B. Xyiose isomerase
C. Chloramphenicol acetyl transferase
D. Aminoglycoside adenyl transferase
25. Primary amino sequence of a protein from five plant species is given to you.
Which of the following tools would you use to know the homology between the
sequences?
A. BLAST
B. TargetP
C. CLUSTALW
D. Primer Express
Friday, 13 March 2015
csir december 2014 result
CSIR is going to announce the December 2014 result will be on last week of march probably
For further information visit CSIR website
Wednesday, 11 March 2015
SVURESET-2015
| |
|
CSIR NET june 2015 date extended to apply online
CSIR has extended the date to submit online application forms from 13 March,2015 to
26 March,2015.
Date of Close of Online Submission: 26-03-2015
Use this opportunity to avoid last minute hurry
Saturday, 7 March 2015
CSIR Lifesciences June 2015
Csir june 2015 notification is rolling . guys it is better to catch the bird early as the Date
of Close of Online Submission 12-03-2015 is reaching.
avoid last minute hurry
We wish you all the best
avoid last minute hurry
We wish you all the best
Friday, 6 March 2015
Success Success Success....
S..
Su..
Suc...
Succ...
Succe....
Succes......
Success..........
Go ... to rock the Show CSIR
Su..
Suc...
Succ...
Succe....
Succes......
Success..........
Success............
Success..............
Success.................
keep on chanting the Success........... Mantra
as a matter of fact today everyone needs success but dont wish for it just work for it. surely you get the success. keep on chatting the success mantra in your work not in your wish surely you will do it.
People don't need Logic they need Magic....
in practical magic wont works but logic only so worship your work. keep trying hard to hit the block to get your success.
CSIR june 2015 practice countdown from today
Get
Set
Go ... to rock the Show CSIR 
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