PART-A
1. Whittaker's five-kingdom classification of living things is based on
A, Cell type only
B. Nutrition only
C. Cell type and level of organization
D. Cell type, level of organization and nutrition type
2. The necessary ingredients for DNA synthesis can be mixed together in a PCR tube. The DNA
polymerase is from Thermus aquaticus and the template is from a human cell. The DNA
synthesized would be most similar to:
A. human DNA
B. human RNA
C. T. aquaticas DNA
D. a mixture of T. aquaticus and human DNA
3. Which is the most stable form of DNA under normal physiological conditions
A. A-DNA
B. H.DNA
C. B-DNA
D. Z-DNA
4. Match the following biological subjects from the Column A with their best description in
Column B and mark the correct answer
Cqlurnn A Column B
L. Agrostology 1. the study of insect pests
M. Palynology 2. the study of pollen grains
N. Entomology 3. the study of autosome
O. Autecology 4. the study of ecology of individual organisms or species
5. study of grasses
A. L-5;M-2;N-1; O-4
B. L-2; M-5; N-l; O-4
C. L-5; M-2;N4; O-3
D. L-5, M-Z;N-I; O-3
5. Root pressure is maximum when transpiration is
A. low and absorption is high
B. high and absorption is also high
C. low and absorption is also low
D. high and absorption is very low
6. All ofthe following are involved in ffanslating information into proteins EXCEpT:
A. rRNA
B. siRNA
C. tRNA
D. snRNA
7. ln nucleotides, phosphate is attached to sugar by
A. Salt bond
B. Hydrogen bond
C. Ester bond
D. Glycosidic bond
8. The bonding of two amino acid molecules to form a larger protein molecule requires
A. the release of a water molecule
B. the release of a carbon dioxide molecule
C. the addition of a nitrogen atom
D. the addition of a water molecule
9. All of the below mentioned amino acids can participate in hydrogen bonding EXCET one -
A. Serine
B. Cysteine
C. Threonine
D. Valine
10. Following its synthesis, an oligonucleotide is dissolved in 1.5 mL of water. you dilute 50 pL
of the oligonucleotide into a total volume of 1000 pL and read the absorbance of the diluied
sample at26} nm. An Azao of 0.264 is obtained. Howmany OD urits axe present in the 1.5
mL of oligonucleotide stock?
A. 0.26
B. A.79
c. 2.64
D. 7.92
1 l. You are given two different protein solutions containing proteins A and B. you forgot to
label those extremely valuable samples. Proteins A anA b exclusively contain alpha/beta
barrel and beta barrel respectively. Which of the following is the- easiest biophysical
technique that can be used to identity protein A and protein B without arnbiguity?
A. Fluorescence spectroscopy
B. CD spectroscopy
C. NMR spectroscopy
D. X-Ray crystallography
12' The first step in two-dimensional gel elecfrophoresis generates a series of protein isoelectric focusing. ln bands by a second itrp, a strip of thiJ gel is turned 90 degrees, placed on another gel containing SDS, and electric current is again applied. In this ,second step'
A' the individual bands become stained so that the isoelectric focus patten can be visualized B' the proteins with similar isoelectric points become firther separated according to their molecular weights
C' the individual bands become visualized by interacting with protein-specific antibodies in ,l the second gel '
D' the proteins in the bands separate more completely because the second electric current is in the opposite polarity to the first current
13' Asymmetric divisions are known to drive plant development. The earliest marker for asymmetry during embryogenesis is
A. abscisic acid
B. auxin
C. gibberellins
D. niric oxide
14' In a given population, only the 'A' and oB' alleles are present in the ABO system: there are no individuals with type 'o' blood or with 'o' alleles in the particular population. If 200 people have type A blood, 7-5 tnve type AB blood, and 25 trave tJrpr B blood, the allelic frequencies of 'p' and .q' in the population are
A. 0.666 and 0.394
B. 0.792 and 0.208
C. 0.833 and 0.167
D. 0.916 and 0.084
15' The major events that occuring Meiosis I (reductional division) are listed below. Which of these is INCORRECT with respect to description of events in that stage?
A' Prophase t: Synapsis of homologous chromosomes and crossing over B' Metaphase-l: Homologous pain of chromosomes line up on the metaphase plate C' Anaphase 1: sister chromatids of each homologous chromosomes move toward opposite poles
D. Telophase l: chromosomes arrive at the spindle poles
16' If the A and B loci are 40 cm apart and an AAbb individual and an aaBB individual mate, the proportion of Ab gametes produced by the F1 individual will be
A. 10%
B. 20%
C. 30%o
D. 40%
17. Eco-friendly biological pesticides are available. Identify one such pesticide among the
following:
A. Bacillus amyloliquifaniens
B. Bacillusthuringiensis
C. Bacillus cerens
D. Bacillus ferriHdans
18. Hallucinogenic alkaloids produced by this fungus can lead to altered behavior, abortion, and
death if grains infected with this fungus axe eaten. Identify, among the following, the fungus
that produces such compounds.
A. Agaricus compestris
B. Aspergiiius jiavus
C. Claviceps Purpurea
D. Penicillium notatum
19. Sulfamethoxazole is a member of sulfonamide group of antimetabolites that are often used as
bacteriostatic antibacterial drugs. Such drugs affect bacterial grourth by
A. Binding to the plasma membrane and disrupt its stnrctrre and perrreability properties
B. Binding to 23S rRNA of large ribosomal subunit (50S) to inhibit peptide chain elongation
during protein synthesis
C. Inhibiting the folic acid biosynthesis by competing withp-aminobenzoic acid
D. Inhibiting DNA-dependent RNA polymerase
20. Which of the following is the CORRECT sequence for the movement of electrons during the
. light-dependent reactions of plants?
A. Water to Pffis to P76s to NADP+
B. Water to P5ss to Pzoo to NADP+
C. Pffis to Pzoo to water to NADP+
D. Poao to water to PTss to NADP+
21. Stokes shift is?
A. difference in absorption
B. difference in wavelength of fluorescence
C. difference in wavelength of absorption and fluorescence emission maxima
D. difference in wavenumber of infrared and frequency
22. Plants growing in shading plants are:
A. Heliophytes
B. Sciophytes
C. Bryophytes
D. Epiphytes
23. The step virus growth cycle' is a technique to understand virus replication. This technique is
given by
A. Emory Eltis & Ma:r Delbruck
B. Walter Reed & Erwin Popper
C. Frederick Twort & Felix Herelle
D. Ma>r Theiler & George Hirst
24.The lowest pH known till now at which bacteria can grow
A. 1.0
B. 0.7
c. 1,7
D. 2.0
25. Serpentine ecosystem is located in
A. Gangetic plain
B. Kutch
C. Andaman & Nicobar
D. Bihar
1. Whittaker's five-kingdom classification of living things is based on
A, Cell type only
B. Nutrition only
C. Cell type and level of organization
D. Cell type, level of organization and nutrition type
2. The necessary ingredients for DNA synthesis can be mixed together in a PCR tube. The DNA
polymerase is from Thermus aquaticus and the template is from a human cell. The DNA
synthesized would be most similar to:
A. human DNA
B. human RNA
C. T. aquaticas DNA
D. a mixture of T. aquaticus and human DNA
3. Which is the most stable form of DNA under normal physiological conditions
A. A-DNA
B. H.DNA
C. B-DNA
D. Z-DNA
4. Match the following biological subjects from the Column A with their best description in
Column B and mark the correct answer
Cqlurnn A Column B
L. Agrostology 1. the study of insect pests
M. Palynology 2. the study of pollen grains
N. Entomology 3. the study of autosome
O. Autecology 4. the study of ecology of individual organisms or species
5. study of grasses
A. L-5;M-2;N-1; O-4
B. L-2; M-5; N-l; O-4
C. L-5; M-2;N4; O-3
D. L-5, M-Z;N-I; O-3
5. Root pressure is maximum when transpiration is
A. low and absorption is high
B. high and absorption is also high
C. low and absorption is also low
D. high and absorption is very low
6. All ofthe following are involved in ffanslating information into proteins EXCEpT:
A. rRNA
B. siRNA
C. tRNA
D. snRNA
7. ln nucleotides, phosphate is attached to sugar by
A. Salt bond
B. Hydrogen bond
C. Ester bond
D. Glycosidic bond
8. The bonding of two amino acid molecules to form a larger protein molecule requires
A. the release of a water molecule
B. the release of a carbon dioxide molecule
C. the addition of a nitrogen atom
D. the addition of a water molecule
9. All of the below mentioned amino acids can participate in hydrogen bonding EXCET one -
A. Serine
B. Cysteine
C. Threonine
D. Valine
10. Following its synthesis, an oligonucleotide is dissolved in 1.5 mL of water. you dilute 50 pL
of the oligonucleotide into a total volume of 1000 pL and read the absorbance of the diluied
sample at26} nm. An Azao of 0.264 is obtained. Howmany OD urits axe present in the 1.5
mL of oligonucleotide stock?
A. 0.26
B. A.79
c. 2.64
D. 7.92
1 l. You are given two different protein solutions containing proteins A and B. you forgot to
label those extremely valuable samples. Proteins A anA b exclusively contain alpha/beta
barrel and beta barrel respectively. Which of the following is the- easiest biophysical
technique that can be used to identity protein A and protein B without arnbiguity?
A. Fluorescence spectroscopy
B. CD spectroscopy
C. NMR spectroscopy
D. X-Ray crystallography
12' The first step in two-dimensional gel elecfrophoresis generates a series of protein isoelectric focusing. ln bands by a second itrp, a strip of thiJ gel is turned 90 degrees, placed on another gel containing SDS, and electric current is again applied. In this ,second step'
A' the individual bands become stained so that the isoelectric focus patten can be visualized B' the proteins with similar isoelectric points become firther separated according to their molecular weights
C' the individual bands become visualized by interacting with protein-specific antibodies in ,l the second gel '
D' the proteins in the bands separate more completely because the second electric current is in the opposite polarity to the first current
13' Asymmetric divisions are known to drive plant development. The earliest marker for asymmetry during embryogenesis is
A. abscisic acid
B. auxin
C. gibberellins
D. niric oxide
14' In a given population, only the 'A' and oB' alleles are present in the ABO system: there are no individuals with type 'o' blood or with 'o' alleles in the particular population. If 200 people have type A blood, 7-5 tnve type AB blood, and 25 trave tJrpr B blood, the allelic frequencies of 'p' and .q' in the population are
A. 0.666 and 0.394
B. 0.792 and 0.208
C. 0.833 and 0.167
D. 0.916 and 0.084
15' The major events that occuring Meiosis I (reductional division) are listed below. Which of these is INCORRECT with respect to description of events in that stage?
A' Prophase t: Synapsis of homologous chromosomes and crossing over B' Metaphase-l: Homologous pain of chromosomes line up on the metaphase plate C' Anaphase 1: sister chromatids of each homologous chromosomes move toward opposite poles
D. Telophase l: chromosomes arrive at the spindle poles
16' If the A and B loci are 40 cm apart and an AAbb individual and an aaBB individual mate, the proportion of Ab gametes produced by the F1 individual will be
A. 10%
B. 20%
C. 30%o
D. 40%
17. Eco-friendly biological pesticides are available. Identify one such pesticide among the
following:
A. Bacillus amyloliquifaniens
B. Bacillusthuringiensis
C. Bacillus cerens
D. Bacillus ferriHdans
18. Hallucinogenic alkaloids produced by this fungus can lead to altered behavior, abortion, and
death if grains infected with this fungus axe eaten. Identify, among the following, the fungus
that produces such compounds.
A. Agaricus compestris
B. Aspergiiius jiavus
C. Claviceps Purpurea
D. Penicillium notatum
19. Sulfamethoxazole is a member of sulfonamide group of antimetabolites that are often used as
bacteriostatic antibacterial drugs. Such drugs affect bacterial grourth by
A. Binding to the plasma membrane and disrupt its stnrctrre and perrreability properties
B. Binding to 23S rRNA of large ribosomal subunit (50S) to inhibit peptide chain elongation
during protein synthesis
C. Inhibiting the folic acid biosynthesis by competing withp-aminobenzoic acid
D. Inhibiting DNA-dependent RNA polymerase
20. Which of the following is the CORRECT sequence for the movement of electrons during the
. light-dependent reactions of plants?
A. Water to Pffis to P76s to NADP+
B. Water to P5ss to Pzoo to NADP+
C. Pffis to Pzoo to water to NADP+
D. Poao to water to PTss to NADP+
21. Stokes shift is?
A. difference in absorption
B. difference in wavelength of fluorescence
C. difference in wavelength of absorption and fluorescence emission maxima
D. difference in wavenumber of infrared and frequency
22. Plants growing in shading plants are:
A. Heliophytes
B. Sciophytes
C. Bryophytes
D. Epiphytes
23. The step virus growth cycle' is a technique to understand virus replication. This technique is
given by
A. Emory Eltis & Ma:r Delbruck
B. Walter Reed & Erwin Popper
C. Frederick Twort & Felix Herelle
D. Ma>r Theiler & George Hirst
24.The lowest pH known till now at which bacteria can grow
A. 1.0
B. 0.7
c. 1,7
D. 2.0
25. Serpentine ecosystem is located in
A. Gangetic plain
B. Kutch
C. Andaman & Nicobar
D. Bihar
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