Sunday, 9 March 2014

AP SET Lifesciences 2012 PAPER II Solved

1. Eukaryotic mRNA can be separated from the total RNA by

(A) Size Exclusion chromatography
(B) Ion-Exchange chromatography
(C) Affinity chromatography
(D) Partition chromatography

2. Which of the following involves the combining of two cells without cell walls from
different plants?
(A) Clonal propagation
(B) Hybridization
(C) Protoplast fusion
(D) Mutant selection

3. Non-polar amino acid residues are found mostly
(A) In the core of proteins
(B) On the surface of proteins
(C) On alpha helix
(D) In no specific region

4. Which of the following organellae does not carry out transcription ?
(A) Nucleus
(B) Peroxisome
(C) Chloroplast
(D) Mitochondrian

5. Given below are two statements
Assertion (A) : Hexokinase and Glucokinase have low and high km towards glucose respectively.
Reason (R) : Glucokinase operates under high glucose concentratian only .
Which of these statements are correct ?
(A) A is correct but R is wrong
(B) R is correct but A is wrong
(C) Both A and R are correct
(D) Both A and R are wrong


6. Given below are two statements. One labelled as Assertion (A) and the other
labelled as Reason (R)
Assertion (A) : Protooncogenes are found in most of the animal cells.
Reason (R) : They are related to viral oncogenes.
In the context of these statements which one of the following is correct ?
(A) A is correct but R is wrong
(B) Both A and R are correct
(C) A is wrong but R is correct
(D) Both A and R are wrong

7. Given below are two statements
Assertion (A) : All eukaryotic mRNAs are capped at 5′ end
Reason (R) : 5′ cap is not essential for efficient mRNA translation
Which of these statements are correct ?
(A) A is correct, R is wrong
(B) Both A and R are correct
(C) A is wrong R is correct
(D) Both A and R are wrong

8. Match the following
I. Nuclear Magnetic Resonance                1. Microwaves
II. Electron Spin Resonance                     2. Radiowaves
III. Raman spectroscopy                           3. UV/Visible light
IV. Spectrophotometry                              4. Infrared waves
      I II III IV
(A) 4 3 2 1
(B) 1 2 4 3
(C) 2 1 4 3
(D) 2 3 4 1

9. Match the following
I. Bt cotton                  1. Improved shelf life
II. Flavr Savr Tomato 2. Improved nutritional quality
III. Roundup Ready    3. Pest resistance Sorghum
IV. Golden Yellow Rice 4. Herbicide tolerance
       I II III IV
(A) 4 2 1 3
(B) 3 1 4 2
(C) 4 2 3 1
(D) 3 2 1 4

10. Viruses that persist in the cell and cause recurrent disease are called
(A) Oncogenic viruses
(B) Latent viruses
(C) Cytopathic viruses
(D) Resistant viruses


11. Assertion (A) : Indirect somatic embryogenesis, embryos are developed without going through
callus formation.
Reason (R) : This is possible due to the presence of pre embryonically determined cells.
(A) Both A and R are true but R is not correct reason for A
(B) A is false but R is true
(C) A is true but R is false
(D) Both A and R are true and R is the correct reason for A

12. In complement cascade the complement
1 activate as follows
I. C1q
II. C1r
III. C1s
IV. C1d
(A) I, II, III, IV
(B) II, III, IV, I
(C) III, IV, II, I
(D) IV, III, II, I

13. Match the following
I. Pyridoxal phosphate 1. Vitamin B

II. Niacin                      2. Vitamin B

III. Thiamin pyrophosphate 3. Vitamin B

IV. Flavin nucleotides 4. Vitamin B

       I II III IV
(A) 1 2 3 4
(B) 2 4 1 3
(C) 4 3 2 1
(D) 3 4 1 2

14. The protein structure is stabilized by
I. Hydrogen bonds
II. Disulphide bonds
III. Glycolidic bonds
IV. Hydrophobic interactions

(A) I and II are correct
(B) II, III and IV are correct
(C) I and IV are correct
(D) I, II and IV are correct

15. Two dimensional electrophoresis involves
(A) SDS-PAGE in 1st dimension and IEF in 2nd dimension
(B) IEF in 1st dimension and SDS-PAGE in 2nd dimension
(C) IEF in 1st dimension and PFGE in 2nd dimension
(D) IEF in 1st dimension and TLC in 2nd dimension


16. Electron Transport chain contains the following components :
I. Cytochrome C-oxidase
II. Succinate/COQ oxidoreductase
III. NADH : COQ oxidoreductase
IV. Coenzyme Q : cytochrome oxidoreductase
Choose correct one of following sequences
(A) III, II, IV, I
(B) II, III, IV, I
(C) I, IV, III, II
(D) IV, III, II, I

17. The correct order of chromatin organization from the following is
I. Nucleosome
II. Solenoid
III. Double stranded DNA
IV. Chromosome
Choose the correct sequence
(A) I, IV, II, III
(B) III, I, II, IV
(C) I, II, III, IV
(D) III, IV, I, II

18. Give the correct sequence of following steps used in Southern hybridization
1. Blotting membrane
2. Restriction digestion
3. Detection
4. Separation by electrophoresis
Choose the correct one
(A) 4, 1, 2, 3
(B) 2, 4, 1, 3
(C) 4, 2, 1, 3
(D) 2, 1, 4, 3

19. According to ICBN Rules the suffix of family name ends with “aceae”, whereas prefix refers to
(A) Specific epithet
(B) Characteristic feature of the family
(C) Generic epithet
(D) Specific character of basic species


20. Pick up the correct combination of the following using valid name and part used
(A) Millets-Sorghum vulgare - Poaceae-  caryopsis
       Pulses-Cajanus cajan-Fabaceae-Cotyledons
       Fibers-Gossypiumherbaceum-Malvaceae-Epidermal hairs of seed 
      Fruit-Mangifera indica-Anacardiaceae-Epicarp and Mesocarp of drupe
(B) Millets-Sorghum vulgare - Poaceae-Caryopsis
      Pulses-Cajanus cajanus-Fabaceae-Cotyledons
      Fibers-Gossypium herbaceum-Malvaceae-Epidermal hairs of seed
      Fruit-Mangifera indica-Anacardiaceae-Epicarp and Mesocarp of drupe
(C) Millets-Sorghum vulgare - Poaceae-Caryopsis
      Pulses-Cajanus cajan-Fabaceae- Cotyledons
      Fibers-Gossypiumherbaceum-Malvaceae-Epidermal hairs of fruit
      Fruit-Mangifera indica-Anacardiaceae-Epicarp and Mesocarp of drupe
(D) Millets – Sorghum vulgare - Poaceae- Caryopsis
      Pulses-Cajanus cajan-Fabaceae-Cotyledons
      Fibers-Gossypiumherbaceum-Malvaceae-Epidermal hairs of seed
     Fruit-Mangifera indica-Anacardiaceae-Mesocarp and endocarp of drupe

21. Assertion (A) : Apospory is not uncommon in some ferns like Pteris
Reason (R) : In ferns some times the prothallus is formed from vegetative parts of sporophytic plant
(A) Both A and R are true, R is the correct explanation of A
(B) Both A and R are true, but R is not the correct explanation of A
(C) Both A and R are not true
(D) A is true, but R is false

22. This biosphere reserve is recently notified by the Government of India
(A) Valley of Flowers
(B) Nanda Devi
(C) Nallamalais
(D) Seshachalam

23. Epiphytes are rare in Delhi because it has
(A) Moist climate
(B) Semi-arid climate
(C) Large amount of factories
(D) Large number of automobiles


24. Arrange the following in progressive manner with special reference to ecological succession in xerosere
I. Funaria
II. Rhizocarpon
III. Poa
IV. Trees
(A) I, II, III, IV
(B) II, I, IV, III
(C) II, I, III, IV
(D) I, III, II, IV

25. Match the following :
1. Aquatic habitats                        I. Sciophytes
2. Community in abundant light II. Xerophytes
3. Community in shade               III. Heliophytes
4. Community in dry conditions IV. Mesophytes
                                                     V. Hydrophytes
       1 2 3 4
(A) V III I II
(B) V IV I II
(C) V III II I
(D) V II I III

26. Pick up the sources of air pollution from the following :
I. Volcanic eruptions
II. Dust storms
III. Domestic sewage
IV. Pulp mills
V. CFMs

(A) I, II and III
(B) I, II, III and V
(C) II, IV and V
(D) I, II and V

27. Which of the following has more redox potential (E0) value ?
(A) Flavo proteins
(B) Cytochrome B
(C) Nicotinamide adenine dinucleotide
(D) Ubiquinone

28. Respiratory quotient (R.Q) value of a simple sugar is
(A) Zero
(B) More than one
(C) Less than one
(D) Equal to one


29. Given below are two statements, one labeled as Assertion (A), and the other labeled as Reason (R)
Assertion (A) : The pronounced decrease in quantum yield at red zone (greater than 680 nm) is termed as red drop. It is also known as Emerson’s effect.
Reason (R) : Discovery of Emerson’s effect has clearly shown the existence of two photosystems.
In the context of the above two statements which one of the following is correct ?
Codes :
(A) A is correct but R is wrong
(B) A is wrong but R is correct
(C) A is correct, R is also correct
(D) A is wrong, R is also wrong

30. Arrange the following compounds in order in which they appear in Krebs cycle. Use the code given below.
I. Succinic acid
II. Fumaric acid
III. Oxaloacetic acid
IV. Malic acid
Code :
(A) III, IV, II, I
(B) I, II, IV, III
(C) II, III, I, IV
(D) I, III, II, IV

31. Which one of the following combination or chromosome number (N) and DNA content (C) is true of the diplotene stage of mammalian oocytes ?
(A) 1N & 2C
(B) 2N & 4C
(C) 2N & 2C
(D) 1N & 4C

32. Assertion (A) : As polyspermy is being slowly blocked, the cortical granules explode and release their contents into the perivitelline space.
Reason (R) : Quick blocking of polyspermy sets in depolarization of the egg plasmalemma and
mobilization of Ca2+ from stores with in the egg.
(A) Both A and R are true and R is the correct explanation of A
(B) Both A and R are true but R is not the correct explanation of A
(C) A is true but R is false
(D) Both A and R are false


33. During early human brain development primary vesicles listed in List I gives rise to derivatives in List II

             List I                           List II
I. Telencephalon                1. Mid brain
II. Mesencephalon             2. Optic vesicle
III. Diencephalon              3. Cerebellum
IV. Myelencephalon          4. Hippocampus
Code :
       I II III IV
(A) 4 1 2 3
(B) 2 1 3 4
(C) 4 2 1 3
(D) 3 2 1 4
34. During neural tube formation in the chick embryo the neural plate involves following events in sequences
(A) Shaping, convergence, folding, elevation, clockwise
(B) Convergence, elevation, folding, shaping, clockwise
(C) Shaping, folding, elevation, convergence, clockwise
(D) Convergence, shaping, elevation, folding, clockwise
35. The order of evolutionary pattern of nitrogen excretion in animals is as follows
(A) Urea, Uric acid, Ammonia
(B) Ammonia, Uric acid, Urea
(C) Urea, Ammonia, Uric acid
(D) Ammonia, Urea, Uric acid

36. Mammalian heterodonty is expressed by a dental formula. Match the dental formula to the organism
List I                                                                            List II
I. 32                                                                           3, 1, 3, 3
                                                                                   0, 0, 3, 3 = 1. Rabbit
II. 28                                                                         1, 0, 2, 3
                                                                                  2, 0, 3, 3 = 2. Man
III. 22                                                                          1, 0, 1, 3
                                                                                   1, 0, 2, 3 = 3. Sheep
IV. 32                                                                      2, 1, 2, 3
                                                                                2, 1, 2, 3 = 4. Squirrel
       I II III IV
(A) 3 1 4 2
(B) 2 1 3 4
(C) 1 4 2 3
(D) 4 1 2 3

37. Assertion (A) : Absorption of the products of carbohydrates and protein digestion are against a concentration gradient, whereas the absorption of the products of lipids are different.
Reason (R) : The end products of carbohydrates, proteins are absorbed into the epithelial cells of intestine is by active transport, whereas the products of lipids are by diffusion.
In the context of the above two statements which one of the following is correct
(A) A is correct but R is wrong
(B) Both A and R are correct
(C) A is wrong but R is correct
(D) Both A and R are wrong

38. The Bohr effect represents an interaction between
(A) O2 equilibrium and Electron dissociation of Hb
(B) O2 equilibrium and Proton dissociation of Hb
(C) CO2 equilibrium and Electron dissociation of Hb
(D) CO2 equilibrium and Proton dissociation of Hb

39. Which one of the following chromosome combinations is expected in an offspring obtained after selfing a plant having AA BB CC DD homologous pairs ?
I. AA
II. BB
III. CC
IV. DD
The correct sequence of the above is
(A) I, III, II and IV
(B) I, II, III and IV
(C) I, IV, III and II
(D) I, III, IV and II


40. Match the most commonly detected blood group systems with probable number of their alleles
       List I List II
Blood Group Alleles
I. ABo          20+
II. MNSc      5
III. P              30+
IV. Rh             4
      I II III IV
(A) 2 1 4 3
(B) 1 2 3 4
(C) 4 3 2 1
(D) 3 4 2 1

41. Assertion (A) : Gene is a biological entitya heritable function detected by observing the effect of a mutation. A gene consists of all the DNA sequences necessary to produce a single peptide or RNA product.
Reason (R) : The gene is single, contiguous protein encoding stretch of DNA.
In the context of the above two statements, which one of the following is correct ?
(A) Both A and R are true and R is correct explanation of A
(B) Both A and R are true but R is not the correct explanation of A
(C) A is true but R is false
(D) Both A and R are false

42. Sex linked genes on the entire X-chromosome of Drosophila melanogaster mapped and found to have a length of
(A) 78 map units
(B) 68 map units
(C) 80 map units
(D) 82 map units

43. What are the two theories that are combined in the synthetic theory of evolution ?
(A) Darwin’s theory of natural selection and Lamarck’s theory of use and disuse
(B) Mendel’s theory of heredity and Heckel’s theory of ontogeny repeats phylogeny
(C) Darwin’s theory of natural selection and Mendel’s theory of heredity
(D) Lamarck’s theory of use and disuse and Heckel’s theory of ontogeny repeats phylogeny


44. Match the various types of isolating mechanisms that prevent gene exchange between population of related species of organisms
             List I                                    List II
I. Habitat Isolation     1. Populations are isolated by different and incompatible behaviour
II. Seasonal Isolation 2. Restricted by differences in structure of reproductive organs
III. Ethological Isolation 3. Populations live in same regions but occupy different habitats
IV. Mechanical Isolation 4. Population exist in the same region but are sexually mature at different
                                                                                                                                                         times
       I II III IV
(A) 1 3 4 2
(B) 3 4 1 2
(C) 2 4 1 3
(D) 3 2 1 4
45. Arrange following fossils in a row leading from more like reptiles to more like mammals
I. Pelycosauria
II. Cyanodonta
III. Therapsida
IV. Mammalia
(A) I, II, III and IV
(B) II, III, I and IV
(C) I, III, II and IV
(D) II, I, III and IV

46. Assertion (A) : The finches beaks evolved to be larger in times of food shortage and smaller in
times of abundance.
Reason (R) : The food supply fluctuated through time according to weather.
(A) Both A and R are true and R is the correct explanation
(B) Both A and R are true, but R is not the correct explanation
(C) A is true but R is false
(D) A is false but R is true


Q.No. 47–50
Read the passage below, and answer the questions that follow based on your understanding of the passage.
DNA replication takes place at a ‘Y’-shaped structure called a replication fork. A self correcting DNA polymerase enzyme catalyzes nucleotide polymerization in a 5′ -to- 3′ direction, copying a DNA template strand with remarkable fidelity. Since the two strands of a DNA double helix are
antiparallel, this 5′ -to- 3′ DNA synthesis can take place continuously on only one of the
strands at a replication fork (the leading strand). On the lagging strand, short DNA fragments must be made by a ‘backstiching’ process. Because the self correcting DNA polymerase cannot start a new chain, these lagging strand DNA fragments are primed by a short RNA primer molecules that are
subsequently erased and replaced with DNA. DNA replication requires the cooperation of many proteins.
These include
i. DNA polymerase and DNA primase to catalyze nucleotide triphosphate polymerization;
ii. DNA helicases and single strand DNAbinding (SSB) proteins to help in opening up DNA helix so that it can be copied ;
iii. DNA ligase and an enzyme that degrades RNA primers to seal together the discontinuously synthesized lagging strand DNA fragments; and
iv. DNA topoisomerases to help to relieve helical winding and DNA tangling problems.
Many of these proteins associate with each other at a replication fork to form a highly efficient ‘replication machine’, through which the activities and spatial movements of the individual
components are coordinated.

47. Helical winding of DNA is removed by an enzyme
(A) DNA polymerase
(B) DNA primase
(C) DNA helicase
(D) DNA topoisomerase

48. Discontinually synthesized lagging strand DNA fragments are sealed by which one of the following enzyme ?
(A) RNA primase
(B) DNA ligase
(C) Single strand binding proteins
(D) DNA polymerase

49. Which one of the following four statements is incorrect ?
(A) Leading strand is synthesized in 5′ → 3′ direction
(B) Lagging strand is synthesized by ‘backstitching’ process
(C) DNA polymerase can directly synthesize new chain of DNA
(D) Antiparallel strands of DNA are separated by helicases

50. Which one of the following four statements is correct ?
(A) Leading strands is synthesized in fragments
(B) DNA fragments synthesis is initiated by DNA primer
(C) Leading and lagging strands are synthesized in different manner because the DNA strands are
antiparallel
(D) DNA polymerase alone coordinates replication of DNA process

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